Rotor Gene 6000 Real-Time PCR Machine Features: • Unique centrifugal rotary format • Uniformity: ± 0.01°C • Resolution: 0.02°C (smallest programmable and reported thermal increment) • 5-Plex (Green, Yellow, Orange, Red, Crimson) fluorescence channels • High resolution melt (HRM) application • Holds 36 x0.2ml tubes, 72 x 0.1ml strip tubes or 100 Gene-Disc • Software: Build-in extensive analysis, graphing and statistical functions. Unlimited use software license for Windows XP, Pentium IV (2GHz) or higher PC. Rotor Gene 3000 Real-Time PCR Machine Features: • Unique centrifugal rotary format • Excitation Source: LED high power diodes, 470nm, 530nm, 585nm and 625nm • Detection Filters: 510nm (FAM), 555nm (JOE), 610nm (ROX) and 660nm high-pass (Cy5) • Holds 36 x0. Wargames 1998 Pc on this page. 2ml tubes or 72 x 0.1ml strip tubes • Software: Build-in extensive analysis, graphing and statistical functions. Use of the machines in the Molecular Biology Facility • The Rotor-Gene machines are in high demand therefore there is an (external website).

Sep 14, 2017 Rotor-Gene Q is a program designed for the QIAGEN Rotor-Gene Q device that enables streamlined analysis for a. Logitech Gaming Software EOSInfo ROBLOX. The Rebellious Ward Pdf on this page. Set-up, programming and analysis: RotorGene 3000/6000, Rotor-Gene Q. Start the Rotor-Gene software. How to start a RotorGene run. Start the Rotor-Gene software. Choose: Advanced/ Perform Last Run and press New. Setup and analysis RotorGene 3000_6000_Q – v4.

Plan ahead, book time slots and stick to them. • Users are responsible for supplying all their own plastic ware and reagents. 0.1ml tubes and Gene-Disc 100 are produced by Corbett especially for the Rotor-Gene. • Save files in your own folder in the 'Rotor Gene Run File' folder in the local disk (C:). The computer is also linked to the network for easy transfer of your data to the server. Tips and addition information • The Rotor-Gene machines support reaction volumes from 5 µl to 100 µl (rotor and protocol dependent). Typical reaction volume is 20µl.

• The use of a normalisation dye, such as ROX reference dye, is optional on the Rotor-Gene. • Many companies supply master mixes for real-time PCR which can decrease the chance of pipetting errors in this highly sensitive method. • If you would like to learn more about the real-time PCR experimental design and data analysis, please contact Dr. Donna Lai, the Molecular Biology Officer. Bioanalyzer, Real-time RT-PCR workshops are conducted occasionally during the year. Please contact Dr.

Donna Lai for details.

QIAGEN's real-time PCR cycler, the Rotor-Gene Q, combines multiple optimized design features to provide the outstanding performance and reliable results that your research demands. Together with optimized QIAGEN kits for real-time PCR, the Rotor-Gene Q enables streamlined analysis for a wide range of applications. Is the new operating and analysis software for life science qPCR applications, with a plug-in concept that lets you add new functionality without affecting established workflows. Explore the to learn more about the Rotor-Gene Q. A human A/T SNP in the AHRR7 gene was analyzed using genomic DNA from wild-type (blue), homozygous mutant (green), and heterozygous (red) samples. Experiments were performed using the Type-it HRM PCR Kit and a Rotor-Gene Q cycler with an HRM channel. Data analysis was performed with the unsupervised mode of Rotor-Gene ScreenClust HRM Software.

A/T polymorphisms (class IV SNPs) are most difficult to discriminate due to minute differences between homozygote alleles (in this example, less than 0.1°C). [ A] HRM raw data, [ B] cluster plot. All pseudo-unknowns were correctly clustered according to genotype. Twofold dilutions of human genomic DNA from 30 ng (10,000 copies) to 0.06 ng (20 copies) were used as a template in real-time PCR. Five replicate reactions were run for each dilution using a self-designed TaqMan® assay for IL1R2 and the Rotor-Gene Probe PCR kit on the Rotor-Gene Q. The average difference in the C T values between all dilutions was 1.07 cycles.

Human genomic DNA was used as template in 72 replicate real-time PCRs using a self-designed TaqMan® assay for BCL2 on the Rotor-Gene Q without ROX normalization. The average C T value was 24.94 with a standard deviation of only 0.05, equivalent to a CV of 0.2%. The Rotor-Gene Q is the only real-time cycler currently capable of deciphering the most difficult class IV SNPs by HRM. Harness the power of HRM using dedicated QIAGEN HRM Kits for applications such as genotyping (see figure ' for data from the Type-it HRM PCR Kit), quantitative methylation analysis (see figure ' for data from the EpiTect HRM PCR Kit), gene scanning, and sequence matching. The Type-it HRM PCR Kit reliably and accurately detects gene mutations and SNPs. The EpiTect HRM PCR Kit enables fast screening and accurate detection of changes in CpG methylation status of bisulfite converted DNA. Unique rotary design for outstanding performance The unique centrifugal rotary design of the Rotor-Gene Q makes it the most precise and versatile real-time PCR cycler currently available (see figure ').